Journal: European Journal of Endocrinology
Article Title: Variable pathological and clinical features of a large Brazilian family harboring a mutation in the aryl hydrocarbon receptor-interacting protein gene
doi: 10.1530/eje-07-0533
Figure Lengend Snippet: Figure 3 Immunohistochemistry of pituitary tumor from index case. (a) The tumor, which stained strongly positive for growth hormone and prolactin (not shown) also had strong positive staining for c-erb. (b) Immunohistochemistry of the pituitary tumor for AIP, showing the reduced AIP staining seen in a region of tumor when compared with a section of normal pituitary.
Article Snippet: Antigen retrieval was performed by microwave boiling in citrate buffer pH 6.0 for two periods of 5 min and one period of 3 min. Immunohistochemistry was performed using a mouse anti-human AIP mAb at a 1:500 dilution (Novus Biological, DBA Italia s.r.l, Segrate, Italy) and a multilink biotinylated antibody and the avidin–biotin peroxidase system according to the manufacturer’s instructions (LSABC kit, DAKO Cytomation, Milan, Italy).
Techniques: Immunohistochemistry, Staining